Rationale: perform plaque assay with plaque 1 to produce first plate with phage

Process:

1. picked a plaque from Melissa’s plate with phage
   1. touched pipette tip to the center of plaque
      1. touched a little bit of the surrounding arthrobacter
   2. swirled tip into tube filled with 70 µL of phage buffer
2. Added 30 µL of phage buffer + phage lysate into o.5 mL arthro and let sit for 15 minutes
3. Made LB agar media for 3 plaque assay plates (control, 2 samples)

   1. Reagents	Amount
      LB Broth	6 mL
      2X TA	7.5 mL
      1M CaCl2	68 µL

4. Poured 5 mL of media into two separate tube for sample plates
   1. Ended up disposing of one of the tubes
5.  Poured Arthro and lysate mixture into a LB agar sample tube
6. Poured contents of tube onto a plate
7. Poured the contents of the remaining LB agar media onto control plate
8. Let solidify for ~15 minutes
9. Incubate for 48 hours at 28 degrees C

Next Steps: calculate titer of lysate and perform another plaque assay to get a higher titer.