November
9
NOVEMBER 5TH AND 7TH- Labs
- NOVEMBER 5TH, 2018
- OBJECTIVE:
- Run a spot test with no contamination
- PROCEDURE:
- Tables were cleaned lamps were lit
- 2mL of lysate was filtered out using a syringe and filter
- Then a large test tube was filled with:
- 4mL LB broth
- 45 𝝁L CaCl2
- 5mL 2x TA
- Then 4.5mL of the 2X TA solution was added to 2 plates, one serving as the control, and the other as the test plate
- Once the 2X TA solution was added, .5mL of arthro was then poured on to the plate
- The plate was then swirled
- Once the plate solidified, 10 𝝁L of lysate was added to its designated sections
- 10 minutes was allowed for the lysate to absorb into the plate
- the plate was then inverted and placed into the incubator
- RESULTS:
- As seen in figure 20, the control was clear of any contaminants, and on test plate were clear of contaminants
- CONCLUSION:
-
- The results seen show that the parts labeled “LEF” and “EB” were native for phage presence while, the spot labeled “SA” was positive for phage presence
-
- NEXT STEPS:
-
- Try to get a webbed plate with new phage
-
- OBJECTIVE:
- NOVEMBER 7TH, 2018
- OBJECTIVE:
- Try to figure out how many 𝝁L of lysate will get a webbed plate
- PROCEDURE:
- Tables were cleaned lamps were lit
- Three test tubes containing .5mL of arthro were obtained
- In the three tubes the following amounts of lysate were added: 20 𝝁L, 40 𝝁L, 80 𝝁L
- In a large test tube the following were mixed
- 8mL LB broth
- 90 𝝁L CaCl2
- 10mL of 2X TA
- Then 4.5mL of the 2X TA solution was added into each test tube of lysate and arthro and was mixed together, then poured on to a plate
- 4.5mL of the 2X TA solution was added to a plate to serve as the control
- 15 minutes was allowed for the plates to solidify
- The plates were then inverted and placed in an incubator
- RESULTS:
- The results can be seen in figure 21
- CONCLUSION:
- The results indicate that there are no phage present on any of the plates.
- NEXT STEPS:
- PCR will be run on the soil to determine if there is a phage
- PCR will be run on the soil to determine if there is a phage
- OBJECTIVE:
