Rationale: The purpose of this lab is to flood a webbed plate to obtain more lysate for future calculations.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an ethanol burner was lit to create an aseptic zone.
2. 5 ml of phage buffer was pipetted onto the webbed plate. The plate was shaken for one hour on the shaker.
3. The lysate was harvested from the plate with a syringe filter and filtered with a 0.22 um syringe filter into the lysate obtained from the last lab.
4. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• 5 ml of phage buffer was used to flood
•  3 ml of lysate was collected from the webbed plate, making a total of 6 ml collected.

Interpretations/Next Steps:

The procedure was complete. The next step will be to perform serial dilutions to calculate the titer of the lysate.

Control and Webbed Plate