Rationale

Redo plaque assay from lab day 20 due to contamination of control plate. Instead of using enriched filtered lysate from donor, used purified filtered enriched lysate of 10^0 for the plaque assay.

Detailed Procedure

1. Took 45 microliters of filtered enriched lysate and added to 400 microliters of Athro. Sat for 15 mins
2. Took 4.2 mL LB Broth, 45 microliters of 1M CaCl2, and 5 mL of 2X TA into a 50 mL vial.
3. Took half of solution from step 2 and poured into control plate
4. Took other half and added solution from step 1 and poured into plaque assay plate
5. Both plates sat for 15 mins and was inverted into the incubator.

Conclusion/Results/Next steps

Spot test results from Soil F was negative, so there will be no need to run a PCR or gel electrophoresis on Soil F. To prevent contamination in the plaque assay, new LB Broth and 2X TA was used and labeled group number on them. In the next lab day, if there is no contamination on the control plate, then I will start calculations to web the plate, since the lysate used was already purified.