10/31/18

Rational:

To record soil G metadata and prepare an enriched lysate for PCR. Also to do a plaque assay with lysate from Claire to determine the titer.

Procedure:

• Cleaned lab desk with CiDecon and ethanol
• Spun down the enriched lysate at 3,000 G for 5 min
• Weighed weigh boat with dry soil

Observations:

• Dry soil with weigh boat- 3.81 g
• Dry soil- 1.82 g
• Percent water- 21.89%
• Sand- 2 mL
  • Percent sand- 20%
• Silt- 4 mL
  • Percent silt- 40%
• Clay- 4 mL
  • Percent clay- 40%
• Soil type- Silty clay

Conclusion:

The soil type for soil G is between clay and clay loam (soil B, C, E, and F), but a little further from soil D (loam). The percent water was also similar to all of the other soil samples. Next lab I will do a plaque assay on the lysate from Claire’s positive sample.