Positive Sample PCR
October 29th, 2018
Shepard Saabye

Objectives and Rationale: Complete PCR on a known sample, to understand if Phage DNA clusters are recognizable at a 10^0 titer.

Procedure:

• Filtered enriched lysate into a microcentrifuge tube
• Boiled Lysate to release DNA from Capsids
• Added 2 microliters DNA to 3 different PCR Tubes, for 3 people
• PCR Tubes contained 12.5 microliters of a 1x Taq Polymerase and dNTP master mix solution
• Added 4 microliters of each primer to its respective tube
• Added 2.5 microliters of DD water to bring total volume of PCR tube to 25 microliters
• Performed PCR on each PCR tube

Results: No results to report.

Analysis and Interpretations: Nothing to Analyze or Interpret.

Future Plans: Perform Gel Electrophoresis on DNA from PCR.