November
2
OCOTBER 29TH AND 31ST- Labs
- OCTOBER 29TH, 2018
- OBJECTIVE:
- To enrich new sample again and run soil metadata
- PROCEDURE:
- Tables were cleaned and lamps lit
- The samples that were incubated last time were contaminated
- The sample was discarded and a new enriched lysate was made:
- Soil was filled to the 2mL mark of a test tube
- Then 10mL of LB broth was added to the tube
- It was shaken fro 15 minutes, before then bing placed into the centrifuge for 10 minutes where it was then spun at 3,000G
- Once the tube being centrifuged was done, a top filter was then used to filter out the supernatant
- Then .5mL of Arthrobactor was added to the filtered supernatant
- The tube was then left in the incubator
- RESULTS:
- No results to report
- CONCLUSION:
- No results to report
- NEXT STEPS:
- Run plaque assay
- OBJECTIVE:
- OCTOBER 31ST, 2018
- OBJECTIVE:
- Plans were changed, and new groups were assigned, where group members will be adopting phages
- Goal —> adopt new plaque and get high titer!
- PROCEDURE:
- Group member calculated the the amount to make a high titer
- High titer amount= 546mL
- Plate was flooded with 8mL phage buffer and left over night
- RESULTS:
- No results to report
- CONCLUSION:
- No results to report
- NEXT STEPS:
- Plate high titer
- OBJECTIVE: