Previous Results:

• The plaque assays of Lysate #5 were examined and all plates (10^0 – 10^-2) were all completely webbed. The 10^0 and 10^-1 plates had almost zero Arthrobacter left on the plate, meaning it was completely covered with plaques.
• The control plate from last lab did not contain any contamination

Objective:

• Collect the phage from the plates using phage buffer
• Use a plate shaker for efficiently collecting the phage

Procedure:

1. Aseptic Zone was created with CiDecon, 70% Ethanol, and an Ethanol burner
2. Plaque assays plated with Lysate #5 were obtained and 6 mL of phage buffer was added to each plate (10^0 – 10^-2)
3. The plates were placed on an electric plate shaker for 75 min instead of allowing the phage buffer to sit for 48 hours to collect phage
4. Then the lysate on the 3 plates was filtered into a 50 mL tube using a syringe and filter. All 3 lysates from the 3 separate plates went into the same 50 mL tube
5. The lysate was then labeled “Lysate #6”

Results:

• The experiment was not completed, therefore there are no results to report. Results will be available during next lab

Next Steps:

• During the next lab Lysate #6 will be diluted out to 10^-4 in order for plaques to be counted for a high titer. If a high titer is obtained, then the experiment will move on in the ordered procedure.