10-31-18

Objective:

A new phage will be adopted from lab colleague  Melissa Leon. Titer of her current lysate and how much lysate will be required to web a plate will be calculated.

Procedure:

Number of plaques formed on plaque assay= 93

volume of lysate used= 30μl

lysate dilution=10^-1

radius of plate= 47.5 mm

radius of plaque= 0.416mm

Ratio of plate and plaque area= π(47.5)^2/π(0.416)^2=1.3036×10^4

Titer=93/30x10x1000=3.1×10^4 pfu/ml

volume for webbed plate= 1.3036×10^4/3.1×10^4=0.4206 ml= 420.6 μl

Analysis and Conclusion

this is a low titer lysate and it will probably be put in for more purification runs do as to acquire a high titer.