Title: Webbed Plate

Date: 29 October 2018

Rationale: After pairing with a lab partner, different methods will be used in order to assist in obtaining a high titer lysate for an already discovered phage.

Procedure: Under an aseptic zone, 2 plates were webbed using a confirmed positive lysate using the following recipe:

• 5 mL 2x Top Agar
• 4 mL LB Broth
• 45 microliters CaCl2

~4.5 mL pipetted into a test tube containing 0.5 mL Arthrobacter culture + either 20 or 30 microliters of positive lysate (one plate was done with 20 microliters and another done with 30 microliters in order to assure a complete lyse and to maximize chances of obtaining a high titer.) THe plates were solidified for 15 minutes and put into the incubator.

Conclusions: In an attempt to raise the titer of the lysate (currently 5.7 x 10^4), the webbed plates were done with double and triple lysate quantities to fully web plates and provide favorable environments for flooding. Assuming one or both plates web completely, they will be flooded in the next lab and the titer checked after a plaque assay.