Rationale

Obtain results from rest of soil metadata procedures and filter enriched lysate. New procedure of heating the lysate instead of syringe filter. One lysate turned green, so syringe filtering on this lab day might help for future testing.

Detailed Procedure

1. Took dropper and inserted 1 mL of enriched lysate into each of the two microcentrifuge vials.
2. Weighed both vials and centrifuged for 2 mins.
3. Poured the vials of the supernatant onto a petri dish lid, used a syringe filter, and inserted into another two separate microcentrifuge vials.
4. Labeled both vials and stored in the fridge.

Conclusions/Next Steps

One of the lysates turned green while the others did not, despite performing the same procedures. To make sure the lysate was filtered properly, group 6 used a syringe filter to ensure all bacteria is filtered out. Lab day 19 took up a lot of time and did not have much time to perform a spot test/plaque assay. Next lab day, group 6 will all perform a spot test together with one control plate.

Soil Metadata Results

• % water= 46.8%