Rationale: After another negative sample it’s back to the drawing board with another sample. I plan to conduct a multi-well enrichment with the next couple of samples and some of my older samples.

Procedure:

1. Filled a 15mL tube with soil up to the 2mL mark then filled with LB broth up to the 12mL mark.
2. Shook with a vortexer for 15 minutes.
3. Spun at 3000g for 5 minutes then filtered with .22μm filter into a 50mL tube.
4. Added 0.5mL of arthrobacter to 50mL tube and placed into shaking incubator.
5. Collected soil metadata for Soil E:
   1. Placed soil in a 50mL conical vial up to the 10mL mark then filled up to the 30mL mark with DI water and added 3 drops of soil dispersion fluid.
   2. Shook vial while holding gloved hand over top for ~1 minute then let sit for 48 hours.
   3. Massed a weigh boat then placed about 3 grams of soil inside. Let sit for 48 hours.
   4. Placed a small amount of soil into vial and added DI water then shook vigorously for 30 seconds then let sit for 2 minutes.
   5. Placed pH paper inside then removed and compared against pH chart.

Observations: The pH of soil E was 6.0. The percent water of the sample was 21.1%. The soil was 6.67% sand, 83.33% silt, and 10% clay.

Conclusions and Next Step: I will have to collect some more soil and then run a multi-well enrichment in the coming lab periods. I will also have to work on putting all the metadata I’ve collected on to the Google Slides spreadsheet.