Rationale:

Re-enriching Soil Sample C to either confirm or deny the presence of plaque in this soil sample, since one member of group obtained plaque from this soil.  

Procedure: 

1. Cleaned the table using Cidecon and 70% ethanol.  
2. Created an aseptic zone by setting up an ethanol burner.   
3. Added 2 mL of Soil Sample C into a 15 mL tube, and added 10 mL of LB Broth into the same tube, and shook for 10 minutes.  
4. Obtained the mass of the solution.  
5. Centrifuged the solution for 10 minutes.  
6. Obtained the solution from the centrifuge and filtered the supernatant using a pipette into a 50 mL tube. 
7. Added 0.5 mL of Arthro to the 50 mL of tube containing the enriched lysate.  
8. Stored the 50 mL tube into shaking incubator until next lab.  

Observations/ Results: 

• Small particles of soil were not filtered out of our enriched lysate due to the lack of filters. 
• Mass of solution before centrifuging: 19.74 grams.
• Enriched Sample amount: 10 mL 

Conclusions/Next Steps: 

Next class, we will prepare a PCR and gel electrophoresis using our newly enriched Soil Sample C in hopes of finding phage.