10/22/18

Rational:

To cut and multiply the DNA in the soil F lysate so that it can be tested for arthrobacter phage. Also to record the results for the percent water and soil texture.

Procedure:

• Cleaned lab desk with CiDecon and ethanol
• Centrifuged the enriched lysate at 3,000 G for 5 minutes
• Put the enriched lysate into a microcentrifuge tube
• Put 4 ML of primer mix 1, 2, and 3 into 3 different tubes
• Added 6.5 ML of DDI water to the three tubes
• Added 2 ML of enriched lysate to the tubes
• Prepare 3 negative control tubes
• Put 4 ML of primer mix 1, 2, and 3 into 3 different negative control tubes
• Added 8.5 ML DDI water to the three tubes
• Put the tubes into the thermocycler

Observations:

• Sand- 3.5 mL
  • Percent Sand- 35%
• Silt- 3.5 mL
  • Percent Silt- 35%
• Clay- 3.0 mL
  • Percent Clay- 30%
• Soil Type- Clay Loam
• Dry soil with weigh boat- 9.01 g
• Dry soil- 6.44 g
• Percent Water- 18.17%

Conclusion:

The percent water in soil F was similar to all of the other soil sample except for soil C. The soil type which was clay loam was the same as soil Band E. The soil type was also similar to soil C (clay) and soil D (loam). Next lab I will be running a gel electrophoresis with the samples that I ran PCR on.