Rationale: run pcr reactions on a gel to see if phage DNA was found and replicated in the soil sample

Process:

1. 0.8 g of agarose were dissolved in 40 mL TBE (Tris/Borate/EDTA) buffer
   1. a microwave was used to heat the mixture in order to dissolve the agarose
2. Once the mixture cooled, the TA added Ethidium Bromide
3. The mixture was poured into a gel tray and left to solidify
4. the solidified gel was then placed in a gel box and submerged in TBE buffer
5. the pcr reactions and ladder were pipetted into the wells according to the following table

   1. lane	sample	amount
      1	RSM & SJ PM1	10 µL
      2	RSM & SJ PM2	10 µL
      3	RSM & SJ PM3	10 µL
      4	Ladder	5 µL
      5	shared	10 µL
      6	shared	10 µL
      7	shared	10 µL

6. the electrodes were connected to the gel box and the gel was run for about 30 minutes
7. Once the yellow marks had moved about 3/4 of way down the gel, the gel was taken out to be photographed with the gel imaging machine

Results:

gel run on 10/22/2018 showing only bands from the ladder

Next Steps:

The results showed no sign of phage DNA in our soil samples so my group decided on collecting a new sample and enriching yet again