Rationale: Collect and gather data on a new soil sample and to also wash and enrich the sample

Procedure:

• A sample of soil was collected and data was gathered
• 2 mL of the soil sample was taken out and transferred to a new vial.
• 10 mL of LB broth was added to the vial and vortexed for 15 minutes.
• Then, the vial was weighed and placed in the centrifuge for 10 minutes.
• The supernatant was transferred and then enriched with 0.5 Arthrobacter.

Results and Analysis:

• Due to the weather, the soil was of a clay-like texture. The soil also had a different composition an inch from the surface of the soil. While the surface had a darker color, the soil an inch in had a light brown color. The tree was located in the square near the main entrance of Teal Residence Hall of Baylor University.
• The diameter was 53 cm.
• The height was 630 cm.
• The average length of the canopy was 265.183 cm.

Due to the clay-like texture of the soil, half of the soil sample was not incorporated into the LB broth and soil mixture when vortexing.

Conclusion:

Due to weather the day the soil was collected, the soil particles stuck together to form a sort of clay. The composition of the soil changed an inch in the soil. Transfer 2 mL of soil and add 10 mL of LB broth. Vortex for 15 minutes or until most of the soil particles are incorporated into the solution with LB broth. After weighing, place the vial in the centrifuge at 10,000 g for 10 minutes. Transfer the supernatant and add 0.5 mL of Arthrobacter.

Future Plans:

In the future (10/29/18), chloroform the sample then filter using syringe and filters.