Rationale

Make the gel for gel electrophoresis. Run a gel electrophoresis to see any sign of plaque from DNA RSM and DNA SJ.

Detailed Procedure

• Took 40 mL of TBE into a flask along with 0.8 g Agarose.
• Microwaved the flask for a bit to heat up.
• Chilled down to warm temperature.
• Added Ethidium Bromide into flask.
• Poured flask contents onto gel tray.
• Let it sat for a few mins to solidify and turned cloudy.
• Put gel tray into gel box and poured TBE just enough to cover the solidified gel.
• Took 10 microliters of Sample 1,2,3 into separate wells.
• Took 5 microliters of ladder into separate wells.
• Took 10 microliters of control (sample 4) into separate gel box
• Ran gel electrophoresis for around 40 mins.
• Took gel off with gloves and place on top of aluminum foil and placed gel onto tray.
• Inserted tray into BioRad Gel Doc EZ Imager to show image of results onto computer.

Conclusion/Next Steps

The image of the gel electrophoresis showed that there is no sign of plaque in both soil sample from SJ and RSM. The next steps is to adopt a plaque from a different group and hopefully continue with picking a plaque.