Rationale

Today we will run gel electrophoresis on the PCR samples created on 10/17/18.

Procedure

• 40 mL of 1X TBE and 0.8 g of Agrose were combined in a flask and microwaved to dissolve. 2 µL of EtBr was added to the warmed solution.
• The mixture was poured onto an electrophoresis tray and was set aside to cool for 20 minutes.
• After cooling, the gel was submerged into 1X TBE until covered.
• 10 µL of each PCR sample and 5 µL of DNA ladder were pippetted into the wells on the gel. Microcentrifuge tubes labeled 1$, 2$, and 3$ were dispensed into wells 5, 6, and 7 respectively.
• The gel electrophoresis apparatus was plugged into a power source, which was then turned on and set aside for 30 minutes.
• The gels were then imaged to display the results.

Observations

After the gels were run, the color produced resembled a light blue while the gels of the other groups produced a more vivid blue.

Conclusion/Next Steps

The results were negative, indicating that new soil must be tested next in pursuit of phage. Previously tested soil may also be re-tested to affirm past discoveries.