10/17/18

Rational:

To filter out soil F after it is collected and enrich it for next lab. Soil metadat will also be collected on soil F for future reference.

Procedure:

• Collected soil from a Post Oak outside of the Business Building
• Cleaned the lab desk with CiDecon and ethanol
• Put 2 ml of soil into a tube
• Added 10 mL of LB broth and shook for 15 min
• Put the tube into the centrifuge at 3,000 G for 5 minutes
• Put 10 mL of soil into a tube
• Added 20 mL of DI water and 3 drops of soil dispersion
• Shook for 30 seconds and then placed the tube under the hood
• Put soil in a pH tube and added DI water
• Shook the tube for 10 seconds and then let it sit for 2 minutes before checking the pH
• Weighed an empty weigh boat and then weighed it with a portion of the soil sample in it
• Put the weigh boat under the hood
• Filtered out the centrifuged soil sample
• Added .5 mL arthro to the lysate
• Put the tube into the shaking incubator at 26 C at 3:40

Observations:

• The tree the soil was collected from had quite a few bare branches and it was lighter than other Post Oaks
• Tree height- 584.8 cm
• Trunk circumference- 41.3 cm
• Canopy width- 170.2 cm
• Soil pH- 6.5
• Empty weigh boat- 2.57 g
• Weigh boat with wet soil- 10.44 g
• Wet soil- 7.87 g

Conclusion:

The soil pH of soil F was the same as soil E which is slightly closer to neutral than the other soil samples. Next lab I will be testing this soil sample for arthrobacter phage. I will also record the results for the soil texture and water percentage.

*Note there was a lot of rain leading up to when soil F was collected so the soil contained more water than usual.

Fig.1.F – This image shows the Post Oak the soil F was collected from. The left side and top of the tree show the most significant absence of leaves.

Fig.2.F – This image show the pH of soil F.