Rationale: To conserve resources, we ran a pcr test on our enriched isolations to check for the presence of phage DNA.

Process:

1. Wash table
2. centrifuged enriched isolation
   1. 5 minutes at 3000 G
3. added ~ 1 mL to microcentrifuge tube
4. gave tube to Lathan to boil
5. Prepared 4 different PCR reactions as shown in the table below

   1. Reagents	Tube 1	Tube 2	Tube 3	Pos. Control
      TAQ Polymerase	12.5 µL	12.5 µL	12.5 µL	12.5 µL
      Primer Mix	4 µL PM1	4 µL PM2	4 µL PM3	4 µL PM1
      DNA RSM	1 µL	1 µL	1 µL	NA
      DNA SJ	1 µL	1 µL	1 µL	NA
      Pos. Control DNA	NA	NA	NA	1 µL
      dd H2O	6.5 µL	6.5 µL	6.5 µL	7.5 µL

6. Gave tubes to Lathan to run PCR cycle in PCR machine

Next steps: Run PCR products on a gel electrophoresis to check for bands that could indicate phage DNA. Also, calculate % sand/silt/clay.