Title: October 15, 2018

Date: 15 October, 2018

Rationale: The previous soil sample tested negative for phage, and can no longer be tested. Due to the weather conditions at the time, the collection of a third soil sample was not possible. By the next lab, a 3rd soil sample will have been collected and will be ready for enrichment and testing.

Conclusions: Both soil samples thus far have yielded conclusive evidence that phage is not present. A third soil sample from a different location will be collected to hopefully find phage. Attached are the results of the 2 most recent plaque assays, one pick resulted in no growth, while the other yielded a negative result due to contamination (the top agar control was contaminated).