Rationale: Re-testing the lysate via plaque assay with a 10^0, 10^-1 and 10^-2 dilution due to no results from the previous plates / contamination. This procedure will be using the same lysate obtained on 10/12

Procedure:

• Created an aseptic zone to prevent contamination by other bacterias
• Obtained the 10^0 lysate from the fridge and obtained four plates
• Created a 10^-1 dilution by pipetting 90μL PB and 10μL 10^0 into a microcentrifuge tube
  • 10^-2 dilution by pipetting 90μL PB and 10μL 10^-1
• Obtained a 50mL conical vial and pipetted 8mL LB Broth and 90μL CaCl2
• Added 10μL of lysate (Respectively) to 0.5mL arthrobacter
• Pipetted 10mL 2XTA into the conical vial and immediately pipetted 4.5mL into the arthrobacter + lysate vials and plated
• Allowed the plates to sit for 15 minutes and then incubated

Observations:

TA control with one spot of contamination

10^0 plate with no plaques

10^-1 plate with one plaque

Next Steps/Conclusion: This plaque assay will hopefully provide positive and measurable results, which can be used to calculate the titer strength. If able to calculate the titer strength and is high enough, then the titer will be prepared for DNA extraction