Rationale: Filtered the PB of the flooded plate to obtain the lysate, and perform a plaque assay to determine the titer (Should be high)

Procedure:

• Create an aseptic zone to reduce the chance of contamination of other bacteria
• Obtained a 22μL syringe and filter and ran the lysate through the filter
  • Filtered the lysate into a conical vial
• Obtained 4 plates for the 10^-1, 10^-2 and 10^-3 plaque assays (+ one TA control plate)
• Created the 10^-1 by pipetting 10μL 10^0 lysate into a micro-centrifuge tube along with 90μL PB
  • Created 10^-2 by adding 10μL 10^-1 to 90μL PB
  • Created 10^-3 by adding 10μL 10^-2 to 90μL PB
• Obtained a conical vial and pipetted 8mL LB Broth and 90μL CaCl2 into the vial
• Added 10μL of lysate (Respective to each dilution) to 0.5mL arthrobacter and let them sit for 15 minutes
• Added 10mL 2XTA to the conical tube and immediately added 4.5mL to the arthrobacter+lysate tube, and plated
• Allowed plates to sit for 15 minutes and incubated
• Cleaned up work area/bench

Observations:

• The filtered lysate is slight yellow in color
• The process of filtering the flooded plate was surprisingly efficient and quick

Next Steps/Conclusion: The next step would be to see the turnout of the plaque assay, and then calculate the titer of the lysate. The lysate should already be high, but performing the plaque assay will confirm. The end goal is to prepare the lysate for DNA extraction.