Rationale: The purpose of this lab is to create a plaque assay from the lysate collected from the flooded plate.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an aseptic zone was created.
2. Top Agar was made for three plates. 6 ml of LB Broth and 67.5 ul of CaCl2 were added to a tube.
3. 10 ul of lysate was added to 0.5 ml of arthrobacter and was allowed to sit for 10 minutes.
4. 7.5 ml of 2x TA was added to the tube and pipetted up and down to mix. 4.5 ml of the top agar solution were added to the arthrobacter and poured onto a plate labeled LIP 10-15-18 Plaque Assay. The rest of the top agar solution was poured directly onto a control plate labeled LIP SS Control 10-15-18.
5. Plates were allowed to sit for 10 minutes and then inverted and stored in the incubator until the next lab.
6. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• Small bubbles were seen on the control plate.

Interpretations/ Next Steps:
The procedure was complete. The next step will be to perform serial dilutions in the next lab.