Rationale

Complete enrichment process of new collected soil and results from soil metadata from new soil.

Detailed Procedure: Soil Metadata

1. Weighed plate and added soil. Recorded both plate and plate +soil
2. Filled soil up to 10 mL mark in a 50 mL vial and filled rest with D.I water up to 30 mL mark.
3. Added 3 drops of soil dispenser solution and shook for 30 secs
4. Left to rest on rack for 48 hours.
5. Pinched soil into pH vial and filled rest with D.I water and shook for 10 secs
6. Left to rest for 2 mins
7. Pinched off 1 inch of pH paper and dipped into pH vial for 45 secs.
8. Recorded pH of soil

Detailed Procedure: Enrichment

1. Filled soil in 15 mL vial up to 2 mL mark
2. Filled LB Broth into vial from Lab Day 14 up to 12 mL mark in aseptic zone
3. Shook using vortex for 10 mins.
4. Weighed vials and centrifuged for 10 mins
5. Syringe filtered supernatant (7.5 mL into 50 mL vial and 2 mL into 15 mL vial)
6. Poured 0.5 mL Arthro into 50 mL vial

Results

• weight of plate= 2.06 g
• weight of plate +soil= 8.00g
• ph of soil= 6
• weight of vial before centrifuge= 20.03g

Conclusions

When 48 hours pass, then the type of soil collected will be determined and will know the percentage of water, sand, silt, and clay. After incubation of enriched lysate, group 6 will be able to do spot test and plaque assay of the each new soil and check to see if there are any presence of plaque.