Rationale: The purpose of this lab is to create a lysate from the flooded webbed plate from soil C.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an ethanol burner was lit to create an aseptic zone.
2. The top agar and phage buffer were spun for 10 minutes in the centrifuge at 5,000 x g.
3. The supernatant was filtered using a 0.22 um syringe filter and stored in the refrigerator until the next lab.
4. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• Filtration process went smoothly

Interpretations/Next Steps:
The procedure was complete. The next step will be to perform serial dilutions and a plaque assay.