Rationale: Complete spot test with new sample isolations to check for plaques.

Process:

1. Set up aseptic system
2. Made LB agar media for 1 control plate and 2 sample plates

   1. Reagents	Control	Sample
      LB Broth	2 mL	4 mL
      2x TA	2.5 mL	5 mL
      CaCl2	23 µL	45 µL
      arthro	NA	1 mL

3. poured 5 mL of LB agar media into each plate and let sit ~5 min
   1. plates must have been too cold because the agar kept sliding around
4. filtered enriches sample with syringe filter, keeping tip clean with ethanol
5. pipetted 5 µL of direct, enriched, and phage buffer (negative control) into designated areas of sample plate and let sit ~10 min
6. incubated for 48 hours
   1. did not invert because of the slippery agar

Next steps: If the spot test is positive, perform a plaque assay to check for plaques again. If the spot test, is negative, dig more dirt!