Rationale: The spot test from the previous class showed no plaques, so group 6 collectively decided to make one last push in soil collection.

Spot test from 10/8/2018 that did not have any plaques

Control plate from 10/8/2018 that shows the agar used was not contaminated

Process:

• Dug dirt from under a red oak tree off campus, approximately 2 feet away from the trunk
• Recorded tree measurements in survey
  • trunk circumference
  • avg crown width
  • tree height
  • tree conditions
  • tree location

Observations:

• had to dig past a lot of mulch to get to actual tree soil

Next Steps:

Wash soil to get direct and enriched isolations. In addition, start collecting soil metadata (%H2O, pH, and sand/silt/clay tests).