10/3/18

Rational:

To collect soil metadata to help explain results in the future. Also to filter soil sample D so that a plaque assay can be performed next lab.

Procedure:

• Cleaned lab desk with CiDecon and ethanol
• Added 2 mL of soil to a tube
• Added 10 mL of LB broth                                                                                                                                                           *  it was later discovered that TA was added intead of LB broth
• Put 2 mL of soil into a tube
• Added 10 mL of LB broth
• Shook the tube for 15 min
• Put the tube in the centrifuge for 10 min
• Added 10 mL of soil to a falcon tube
• Added 20 mL DI water
• Added 3 drops of soil dispersion
• Weighed empty weigh boat- 2.46 g
• Weighed weigh boat with wet soil- 3.69 g
  • Weight of wet soil- 1.23 g
• Placed weugh boat under hood
• Put soil in a pH vial and added DI water
• Shook for 10 sec
• Let sit for 2 min
• Checked pH of the soil
  • pH- 6
• Shook soil in the falcon tube for 30 sec
• Placed under hood
• Filtered soil D with a top filter
• Placed in a shaking incubator at 26 C at 3:30-2:30 10/5
• Added arthro to the enriched lysate before placing back in the shaking incubator
• Cleaned lab desk with CiDecon and ethanol

Conclusion:

The soil sample had a pH of 6 like the two soil samples before. Next lab I will do a plaque assay on soil D in order to check for arthrobacter phage. I will also record the results for the soil texture/type and percent water for soil D.

Fig.6.D – Shows the pH of soil sample D is about 6.