Rationale: Plates from last week were plaque-less yet again, so we decided to find new soil from another red oak.

Spot test from 9/26/2018. No plaques found.

Process:

• Dug dirt from under a red oak tree, approximately 2 feet away from the trunk
• Recorded tree measurements in survey
  • trunk circumference
  • avg crown width
  • tree height
  • tree conditions
  • tree location
• In the lab, started soil metadata tests
  • % H2O
    • mass of weigh boat = 2.32 g
    • mass of wet soil = 4.45 g
  • sand/silt/clay
    • filled falcon tube with ~4 mL soil
    • added DI to 12 mL mark
    • added 3 drops soil dispersion
    • shook for 30 seconds
    • let sit for 48 hours

Next Steps:

Wash soil to get enriched and direct sample isolations.  Measure pH of soil and calculate the % H2O and sand/silt/clay.