October 5

OCTOBER 1ST AND 3RD- Lab

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  • OCTOBER 1ST, 2018
    • RESULT ANALYSIS AND NEW SOIL COLLECTION
      • RESULTS: (RESULTS FROM 9/26 WERE NOT VIEWED UNTIL 10/1)
        • As seen in Figure 14 the control and plaque assay plate were both clear of contaminants
        • The plaque assay seen in the lower plate on the picture is negative for phage presence 
      • CONCLUSION: 
        • The plaque assay was negative for phage presence therefore, it can be concluded that the soil sample does not contain phage. 
        • NEXT STEPS: 
        • A new soil sample will obtained and a new plaque assay will be conducted with the sample 
      • OBJECTIVE: 
        • Obtain a new soil sample
      • PROCEDURE: 
        • A new soil sample had to be collected, and a tree was located outside of Mclane stadium
        • A shovel was used to collect to dirt 2 feet away from the base of the tree, then the tree’s height, canopy diameter, and trunk circumference were measured 
        • Data for the tree will be entered into tree data survey 
      • RESULTS: 
        • No results to be recorded 
      • CONCLUSION:
        • Nothing to conclude 
      • NEXT STEPS: 
        • To wash new soil sample
  • OCTOBER 3RD, 2018
    • SOIL WASHING AND FILTRATION
      • OBJECTIVE: 
        • To wash and filter soil to prepare for plaque assay 
      • PROCEDURE: 
        • Tables were cleaned and lamps were lit
        • A test tube was filled to the 2mL mark with soil, which then had 10mL of LB broth added to it 
        • The tube was then shaken for 15 minutes
        • The tube was then centrifuged for 10 minutes at 3,000 RPMs
        • A falcon tube was then filled with 10mL of soil, and was then filled to the 30mL mark with DI water
          • 3 drops of dispersion fluid were then added to the tube, and the tube was then shaken for 30 seconds, then was left to settle
        • Once the tube being centrifuged was done, a top filter was then used to filter out the supernatant 
          • Then .5mL of Arthrobactor was added to the filtered supernatant 
          • The tube was then left in the incubator 
    • RESULTS: 
      • Waiting for metadata results 
    • CONCLUSION:
      • Waiting for metadata results 
    • NEXT STEPS: 
      • Plate plaque assay 


Posted October 5, 2018 by laurenfoley_foley1 in category Lauren Foley

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