Rationale: Perform spot test with new direct and enriched isolations to check for the presence of phages

Process:

1. set up aseptic system
2. made LB agar broth for sample plates and a control plate

   1. Reagents	Control	Sample
      LB Broth	2 mL	4 mL
      2x TA	2.5 mL	5 mL
      CaCl2	23 µL	45 µL
      arthro	NA	1 mL

3. poured 5 mL of LB agar broth into each plate and let sit for ~10 min
4. used syringe filter to filter enriched sample
5. pipetted 5 µL of direct isolation, enriched isolation, and phage buffer (negative control) into designated areas of sample plate and let sit for ~10 min
6. put plates in incubator for 48 hours

soil metadata:

1. % H2O:
   1. mass of water = 0.49 g
   2. mass of wet soil = 6.01 g
   3. 0.49 g / 6.01 g * 100% = 8.15% H2O
2. sand/silt/clay
   1. total volume = 2.75 mL appr.
   2. sand = 1 mL appr.
   3. silt = 1 mL appr.
   4. clay = 0.75 mL apprx.

1 / 2.75 * 100% = 36.4% sand

1 / 2.75 * 100% = 36.4% silt

0.75 / 2.75 * 100% = 27.2% sand

soil metadata: sand, silt, and clay layers

Next steps: If the spot test is positive, perform a plaques assay with enriched isolation to check for plaques again. If the spot test is negative, look for a new soil sample outside.