9/19/18

Rational:

Plaque assay 2 soil B showed not plaque or contamination. A new soil samplw was collected and filtered to prepare for a new plaque assay to check for phage.

Procedure:

• Found a post Oak behind East Village for Kathryn to use
• Found a chesnut Oak in Earle court yard
  • Trunk circumference- 42.4 cm
  •  Canopy length- 238.6 cm
  • Tree height-
• Collected soil 2 ft from the trunk
• Put 2 mL soil C into tube from the sample collected
• Put 10 mL LB broth in the tube and shook for 15 minutes
• centrifuged the tube at 5,000 G for 5 minutes
• Filtered the sample and added 0.5 mL arthro
• Put in shaking incubator at 4:10 (26 C)
• Cleaned desk with CiDecon and ethanol

Conclusion:

Next lab metadata will be collected on soil C that can be looked at later to help explain results and trends. On monday a new plaque assay will be preformed which will test for the presence of arthrobacter phage in soil C.

Fig.7.B – Plaque assay 2 for soil B shows no sign of a                 Fig.8.B – Control plate shows no sign of contamination. plaque or contamination.

Fig.1.C – Shows the tree that soil sample C was taken                 Fig.2.C – Shows a leaf from the Chesnut Oak the soil      from.                                                                                                       sample C was taken from.