Rationale: My lab group performed a spot test and plaque assay with soil from the previous red oak tree and got negative result. We wanted to fins a new red oak tree to test the soil from.

Figure 1: Results of the spot test from the previous class. No plaques found

Process:

1. found a red oak in the field outside of the SLC and dug soil samples from the roots
2. Started % H2O metadata analysis:
   1. mass of wet soil in weigh boat = 8.38 g
   2. let the wet soil sit for 48 hours

Next steps:

wash soil to get enriched and direct isolations. Continue to analyze soil metadata by taking soil pH, weighing the dry soil, and setting up a sand/silt/clay soil dispersion.