September
21
Soil C Metadata
9/21/18
Rational:
To get metadat such as percent soil, silt, and clay, percent water, and pH to later help explain any results and trends found.
Procedure:
- Cleaned the lab dest with CiDecon and ethanol
- Added 10 mL of soil C to a falcon tube
- Added DI water to the 30 mL mark
- Added 3 drops of soil dispersion
- Shook for 30 seconds and then placed it under the hood
- Weighed empty weigh boat- 2.32 g
- Weighed weigh boat with wet soil- 5.62 g
- Weight of wet soil- 3.3 g
- Placed soil under the hood
- Put soil in pH vial
- filled with DI water
- Shook tube for 10 seconds
- Let sit for 2 minutes
- Checked pH with pH paper- pH of 6
- Cleaned lab desk with CiDecon and ethanol
Conclusion
Next lab a plaque assay will be preformed to test for arthrobacter phage in soil C. The pH of soil C was the same as the pH of soil B and the rest of the metadata will be compared next lab when the results are recorded.
Fig.3.C – Shows the pH of soil C is about 6