9/21/18

Rational:

To get metadat such as percent soil, silt, and clay, percent water, and pH to later help explain any results and trends found.

Procedure:

• Cleaned the lab dest with CiDecon and ethanol
• Added 10 mL of soil C to a falcon tube
• Added DI water to the 30 mL mark
• Added 3 drops of soil dispersion
• Shook for 30 seconds and then placed it under the hood
• Weighed empty weigh boat- 2.32 g
• Weighed weigh boat with wet soil- 5.62 g
  •  Weight of wet soil- 3.3 g
• Placed soil under the hood
• Put soil in pH vial
• filled with DI water
• Shook tube for 10 seconds
• Let sit for 2 minutes
• Checked pH with pH paper- pH of 6
• Cleaned lab desk with CiDecon and ethanol

Conclusion

Next lab a plaque assay will be preformed to test for arthrobacter phage in soil C. The pH of soil C was the same as the pH of soil B and the rest of the metadata will be compared next lab when the results are recorded.

Fig.3.C – Shows the pH of soil C is about 6