Rationale

Complete enrichment process of new collected soil and results from soil metadata from new soil. Label group number on LB Broth and 2X TA jars to prevent future contamination.

Detailed Procedure: Soil Metadata

1. Filled soil up to 10 mL mark in a 50 mL vial and filled rest with D.I water up to 30 mL mark.
2. Shook for 10 secs and added soil dispenser solution.
3. Left to rest on rack for 48 hours.
4. Pinched soil into pH vial and filled rest with D.I water and shook for 10 secs
5. Left to rest for 2 mins
6. Pinched off 1 inch of pH paper and dipped into pH vial for 45 secs.
7. Recorded pH of soil

Detailed Procedure: Enrichment

1. Filled LB Broth into vial from Lab Day 7 up to 12 mL mark in aseptic zone
2. Shook using vortex for 15 mins.
3. Weighed vials and centrifuged for 5 mins
4. Syringe filtered supernatant (8 mL into 50 mL vial and 1 mL into 15 mL vial)
5. Poured 0.5 mL Arthro into 50 mL vial

Results

• weight of plate + soil after 48 hours= 6.48 g
• ph of soil= 6
• weight of vial before centrifuge= 19.75 g

Conclusions

After 48 hours, I will be able to determine what type of soil was collected. After incubation of enriched lysate, group 6 will be able to do spot test and plaque assay of the new soil and check to see if there are any presence of plaque.