• SEPTEMBER 10TH, 2018
  • SOIL WASHING AND METADATA
• OBJECTIVE: To wash new soil samples collected and to perform metadata on the soil as well
• PROCEDURE:
  • Tables were cleaned 
  • Next soil samples were used to fill a test tube to the 2mL mark, filled to the 12mL mark with LB, and were then shaken and centrifuged for 15 minutes
  • The tubes were then taken to spin @10,000G for 5 min 
  • During that time 10mL of soil was added to a conical vile 
  • DI water was added to the vile until it hit the 30mL mark
  • Then 3 drops of soil dispersion fluid was added to the vile, where it was then shaken for 30 seconds 
  • The test tube of soil and broth was taken to a fume hood where the supernatant was poured into a top filter, where 10mL was filtered out 
  • Then the 10mL filtered supernatant  then has .5 mL of arthrobactor added to it and was left to incubate
  • Wet soil sample was weighed out and then left to dry 

– NEXT STEPS: Conduct spot test

• • SEPTEMBER 12TH, 2018
    • SPOT TEST
    • OBJECTIVE: Conduct a spot test without any contamination 
    • PROCEDURE: 
      • Tables were cleaned 
      • Dry soil sample was weighed out 
        • Plate without soil: 7.20g
        • Wet soil: 10.02g
        • Dry Soil: 9.45g
        • Water: 0.57g 
        • %Water: 
    • Then meta data was analyzed: 
      • • %sand: 54.02%
        • %clay: 26.44%
        • %silt: 19.54%
    • My sample was then masses and a “spin buddy” was found
    • The sample was spun at 3000G for 5 min
    • Then a syringe was used to push the spun supernatant through a .22𝝁m filter out 
    • Then a test was made with the following:
      • • 6mL LB
        • 1.5mL Arthro
        • 7.5mL 2X TA
        • 67.5𝝁L of 1M CaCl 
• 5mL of the contents of test tube were pipetted onto 3 plates and left to sit for 15 min
• After 15 min .5𝝁L of lysate of was pipetted onto plate
• Was left to incubate
• NEXT STEPS: wait to see results from spot test