Rationale

Make plaque assay and record results from Lab day 5

Detailed Procedure

1. Since I was the only one in my group without a direct lysate, I had to make a plaque assay while the rest did a spot test. (therefore I had to make my own control)
2. Add water to help balance my partner’s weight= final enriched weight= 22.38 g
3. Filtered enough enriched lysate into a 15 mL vial.
4. Took 10 microliters of enriched lysate and added to 0.5 m Arthro vial
5. Mixed it well and let sit for 10 mins
6. During that time, took 2.0 mL LB Broth, 23 microliters of 1M CaCl2, and 2.5 mL 2X TA and mix well through pipetting in a separate 50 mL vial
7. Took enriched lysate + Arthro mixture and added to the 50 mL vial
8. Mixed one more time through pipetting
9. Poured mixture onto a plate label “Plaque Assay B”
10. Repeat step 6 for control onto a new plate labeled “Plaque Assay Control”
11. Let both plates sit for 15 mins

Detailed Procedure: Sand, Silt, Clay+ Metadata results

1. Record total mL in tube
2. Record how many mL of sand, silt, clay, and calculate the %

Observations/Results

• Sand= 50%
• Silt= 43.75%
• Clay= 0.0625%
• Soil + petri dish= 5.75 g

Next Steps

• Wait for results from plaque assay and see if there’s any presence of plaque.
• Results and comparison to rest of class will help resolve/eliminate any variables or questions we are trying to solve

enriched lysate w water

control vs exp

after 48 hours