Rationale:

• Make enriched lysate of soil B, find pH of soil and start soil metadata to find future calculations of sand, silt, and clay.

Detailed Procedure: Enriched Lysate

1. Took vial from Lab day 4 and added LB Broth up to 12 mL mark
2. Shook vial for 10 mins
3. Weigh vial= 19.04 g
4. Centrifuge for 5 mins
5. Syringe filter 7.5 mL of supernatant (did not have enough for direct)
6. Add 0.5 mL Arthro to vial

Detailed Procedure: Metadata

1. Add ~10 m of soil in a 50 mL vial.
2. Add water up to 30 mL mark
3. Shook vial for 30 secs
4. Add soil disperse solution to it and mix for a bit
5. Let sit for 48 hours
6. Took some soil and weigh it with petri dish
7. Let sit for 48 hours to dry

Detailed Procedure: pH

1. Took pinch of soil in a small pH vial and filled the rest with water
2. Shook for a bit and rip off 1 inch of pH paper
3. Dip paper for 45 secs and compare color
4. Recorded ph was green= 7.0

Observation/Results

• petri dish weight= 2.35 g
• petri dish weight + soil= 6.26 g
• after this, I ran out of all of my soil, so I have no more left to test if needed in the future.

Next steps

• calculate sand, silt, clay
• weigh petri dish when soil dries
• plaque assay with enriched because I have no direct lysate
•