September
7
SEPTEMBER 5TH 2018- Lab
- SEPTEMBER 5TH, 2018
- QUESTION DEVELOPMENT AND PLAQUE ASSAY ANALYSIS
- OBJECTIVE: Develop a question for the class to investigate for the semester, and to analyze the results of the plaque assay conducted
- POSSIBLE QUESTIONS:
- Comparison of concentration of arthrobactror in:
- Different species of oak?
- White vs red oak?
- in transplanted trees vs. naturally grown trees?
- Could compare old trees to young trees bases on girth of tree?
- Comparison of diseased oak vs. healthy oak?
- Trees/oaks in an area affected by fire vs. trees/oaks in “normal” environments?
- Recent fire near Texana?
- Arthrobactor has been known to be known to breakdown and metabolize pesticides so:
- My table has decided to test oaks in Cameron park that are untreated with pesticides and compare it to oaks at Baylor that have all been exposed to some from of pesticides
- Comparison of concentration of arthrobactror in:
- RESULTS:
- The plaque assay ,shown in figure 6, was negative for any bacteriophages
- NEXT STEPS:
- Since the spot test and plaque assay were both negative
the next step is to collect soil from oaks (species of oak has yet to be determined) from Cameron park and soil from trees treated with pesticides at Baylor and compare the concentration (or presence) of phages
- Since the spot test and plaque assay were both negative
