09/05/2018

Soil Collection Sample B

Objectives:

• Collect new Soil Sample
• Create a research question
• Collect metadata for new soil sample

Pre-lab analysis:

Analyzed the plaque assay from 08/29/2018. The plate was negative. The plates with lysate of soil sample from the same location was also negative. Conclusion, there are no bacteriophages in that soil sample.

Materials Required: Ziploc bag, sharpie for labelling, 15 ml conical vial, tool for digging and transferring soil, meter tape and electronic device for filling out the metadata survey

Procedure:

1. We were assigned new group members and our designated spots.
2. The research question was decided by the 6 people at the table. We decided to test the difference in the presence of bacteriophages in the soil of live oak and red oak.
3. My side of the table will be collecting a sample from a live oak.
4. We found our live oak right of the beach of the BSB
5. We collected one leaf sample from the tree ( 1 per group member)
6. We collected the metadata for the tree and recorded it in the survey
7. We collected soil in the Ziploc bags.
8. We then transferred 2 ml of soil to a 15ml conical
9. We put the leaf sample, soil sample and the 15ml conical vial in the bag.
10. Store the samples in the fridge to prevent change in the soil before the washing and enrichment process.

Pictures:

Analysis: no real analysis can be made for what we did today. the research question the table decided upon : ” Is there a difference in the presence of bacteriophages in the soil of two different species of oak ( live and red oak )? ”

Future notes: remember the adjustment for protocols for the washing and enrichment process. Develop better communication with peers to speed up process of decision making and planning.