Lab goal: spot direct and enriched samples on an LB plate to test for plaques

Process:

1. Made LB top agar
   1. Decided to make 4 plates worth of agar media in one 50 mL tube so we started by putting 18 mL of LB broth into the 50 mL tube
   2. After this step we decided to separate our control media so we moved 4.5 mL of the LB broth into a new 50 mL tube
   3. The table below shows the amounts of reagents added to the two tubes
   4. 
      

      Reagent	Control Tube	Plate Agar
      Artho	NA	1.5 mL
      LB Broth	4.5 mL	13.5 mL
      2x TA	5.0 mL	15 mL
      1M CaCl2	45 µL	135 µL

2. Used a pipette to put 10 mL of agar into a plate for each group member (Rachel, Lauren, and Lily)
3. Poured all of the control tube into the control plate
4. We let the plates sit for about 10 minutes
5. Using a sterile syringe and a syringe filter, filter about 2 mL of the enriched isolated sample into a microcentrifuge tube
6. Used a micropipette to spot plate with 4.4 µL of:
   1. Filtered enriched isolated sample
   2. Direct isolated sample
   3. Phage Buffer (negative control)
7. Started incubating the plates (not inverted) at 4:14 PM