Lab goal: wash soil samples collected from section 8 and produce a direct isolated sample and an enriched isolated sample.

Process:

1. Cleaned lab bench with CiDecon and 70% ethanol
2. Using aseptic techniques, added LB broth to the 35 mL mark of the 50 mL tube containing approximately 15 mL of soil
3. shook and vortexed the tube for 15 minutes
4. Added about 1/2 mL of water to get a final weight of 46.59g
5. Centrifuged the tube with the rest of the class
6. Used a bulb pipette to transfer supernatant from the centrifuged tube to a filter
7. After filtering, poured about 3.5 mL of the filtered product into a 15 mL tube leaving about 8 mL in the 50 mL tube
8. Added 0.5 mL of artho to the 50 mL tube
9. Labeled the 15 mL tube as direct isolated sample and stored it in the fridge
10. Labeled the 50 mL as enriched isolated sample and left it in the shaking incubator