Rationale: Perform a plaque assay to double check for bacteriophages in the soil samples

Results and Observations from 8/27/2018 spot test:

Figure 1: Contamination found on spot test plate. Results are not viable.

Figure 2: LB agar media control plate. The plate was clean and showed no signs of contamination.

Process:

1. Made top agar for 3 plaque assay plates (one for me, Lauren, and Lily)
2. The table below shows what we added to the media
   1. 
      

      Reagent	Amount
      LB Broth	8.0 mL
      2x TA	10.0 mL
      1 M CaCl2	900 µL

3. Added 10 µL lysate to 0.5 mL artho
4. Let the solution stand for approximately 20 minutes
5. Pipetted about 1 mL of LB agar onto 1/4 of a shared controlled plate
6. Separated the remaining agar into 3 separate 50 mL tubes for each lab partner (about 6 mL in each)
7. Added artho + lysate solution to my tube
8. Poured entire contents onto sterile plate (that already had LB agar on it)