Rationale:

Filter the Direct Isolation lysate

Procedure:

• Take the Direct Isolation out of the refrigerator and get a new vial.
• Fill the new vial with water with the same mass as the Direct Isolation.
• Place the tubes directly across from each other in the centrifuge and allow it to spin from 5-10 minutes.
• Take the Direct Isolation out of the centrifuge and place it in the test tube rack in the refrigerator.

Results and Analysis:

• Upon receiving my direct isolation, I noticed that the sample was already separated. Some minutes later, without me shaking the tube, the sample became evenly mixed again.

Conclusion

• I did not fulfill the purpose of this lab which was to filter the direct isolation. However, I did complete half of the procedure which was to put it in the centrifuge to separate the phage from the dirt and bacteria.

Future Plans

• In the future (8/28/28), I plan to finish filtering my Direct Isolation lysate. I also plan to conduct a spot test in which the filtered Direct Isolate will come in play.