August
31
AUGUST 29TH 2018- Lab
- AUGUST 29, 2018
- PLAQUE ASSAY TEST AFTER NEGATIVE SPOT TEST
- OBJECTIVE: Conduct a plaque assay with little to no contamination, and as a class also develop an over arching question to investigate this semester
- PROCEDURE:
- Tables were sanitized and lamps were lit to create an aseptic environment
- 4 plates were obtained (one plate was used as control for my group and 3 other groups as seen in figure 5)
- Next, in a culture tube the following were mixed:
- .5mL arthro
- 10𝝁L of lysate
- The lysate and arthro then sat for 15 minutes
- Then a 50mL tube was filled with:
- 8.0 mL LB broth
- 10.0 mL of TA
- 90𝝁L 1M CaCl2
- Then 4.5 mL of the TA solution was pipetted into each group member’s culture tube
- The culture tube was then poured onto a plate, where it sat for 10 minutes to solidify, where it was inverted and put in the incubator
- NEXT STEPS: Wait for results from plaques assay and continue to brainstorm a testable question to research
