August
31
AUGUST 22ND 2018- Lab
- LAB NOTE BOOK INSTRUCTIONS:
- Date/Title/Rational
- Description of procedure (what you actually did, NOT what you were told to do)- storage
- Observations/Results/Data
- AUGUST 22, 2018
- CLEANSING AND SEPARATION OF OAK TREE DIRT
- OBJECTIVE: To clean and separate out the soil sample, into a direct and enriched sample
- PROCEDURE:
- The tables were sanitized and lamps were lit to create an aseptic environment
- A 50mL test tube was filled to the 15mL with soil that was collected 48 hours prior to the lab in section 8, from a healthy oak tree, seen in figure 1
- Then LB broth was added to the tube util it reached the 35mL mark
- The tube was then shaken by hand and vortex for 15 minutes
- The tubes were then taken to the centrifuge when they centrifuged as a class
- The contents of the soil had separated out into supernatant in the top and a solid in the bottom
- The supernatant was then pipetted into a filter where pressure was applied to it to force it through the .22 𝝁m and dripped into a 50mL tube as seen in figure 2 (must be conducted in fume hood)
- The supernatant was filtered until 10mL was filtered out
- The 10mL of the filtered supernatant had .5mL of Arthro added to it (This became the enriched sample), and the sample was then placed in the fridge
- The excess unfiltered supernatant was put into a separate test tube and placed in the fridge
- NEXT STEPS: Conduct Spot test with samples + filter out direct sample
