Detailed Procedure

1. Shake the bag of soil into vial Soil A. It was around 15 mL of lumpy soil.
2. Add the LB Broth to my vial at the 35 mL mark. (I had to use two bottles of LB Broth since the first one did not fill up to the 35 mL mark.)
3. Shake the vial by hand and vortex for 15 mins.
4. Weighed the tube= 52.21 grams
5. Centrifuge for 5 mins.
6. Tube is now separated  from soil pellet and supernatant. Looks very cloudy.
7. After two days, I filtered it out.
8. To filter, use a dropper to put the supernatant into the tube top filter.
9. After 23 mins, I poured 12.5 mL of filter supernatant into a 50 mL vial.
10. Went to aseptic zone to pour arthro into the 50 mL vial labeled as Enriched A
11. For the rest of supernatant that didn’t filter, put it in a centrifuge for 5 mins  in a 15 mL vial.
12. Used a syringe filter for 30 mins and got 4.5 mL of Direct Isolation A into a 15 mL vial.

Thoughts

• As I put the soil in the tube, it came out lumpy and slightly wet. Perhaps this might have affected the result of a cloudy appearance when put in the centrifuge
• Some large particles in the soil may have clogged up the filtering process.
• If the soil was dry, the supernatant filtering might have been easier.