8/22/18

Filtration Soil A

Rational:

The soil sample will be filtered out in order to get a sample containing only bacteriophages in the broth. This will allow me to test for the presence of bacteriophage that infects arthrobacter since the bacteriophage will eventually be placed in and environment containing only arthrobacter (no other bacteria).

Procedure:

• Cleaned the lab desk with CiDecon and 70% ethanol
• Used burner to set up an aceptic zone and added 40 mL of broth to the soil sample A (weight 60.07 mg)
• Shook the tube for 15 min to break up the soil sample A
• The soil sample was then matched with another sample with a similar weight and centrifuged for 5 min at 3,000 G
• The liquid at the top of the sample was then filtered through a top filter
• The filtered lysate was then separated into a direct isolation sample of 8.3 mL and an enriched sample of 10 mL (with arthrobacter added)
• Lab desk was cleaned with CiDecon and ethanol

Future Steps:

I will do a spot test and later a plaque assay using the direct isolationa and enriched lysate in order to test for the presence of bacteriophage that infect arthrobacter in the soil sample A

fig.1 – shows the layers seen in the soil sample                                                                                                                          after the sample was centrifuged