Rationale: To create a direct lysate sample for the experiment from the soil, without adding arthrobacter.

Scientific Research Question: Does the presence of arthrobacter bacteriophage appear more dominant in one oak tree species than others?

Description of Procedure:

1. The sample of supernatant that had not yet been filtered was spun in a centrifuge for 5 minutes at approximately 4000 x g.
2. This sample was then put into a 0.22 um filter using a pipette and filtered. Approximately 7.35 mL of lysate was obtained from filtering the supernatant.
3. This lysate was then transferred into a 15 mL tube, not in an aseptic zone. This sample will be refrigerated until Monday and will eventually be plated.
4. The used materials were then properly disposed of and the work station was cleaned.

Observations/Results/Data:

Observations:

• After being spun in the centrifuge a second time, the filtering process was much quicker.
• The supernatant was cleared than it had been before the second spin.
• The transferring of the direct lysate to a smaller container was not done in an aseptic zone.
• Approximately 7.35 mL was obtained of direct lysate.

Results:

The procedure from Wednesday was completed, with the direct lysate sample having now been obtained. This sample is approximately 7.35 mL.

The sample will be stored in the refrigerator.

The tube was labeled LIP 8-24-18 Direct Lysate

Interpretations/Conclusions/Next Steps:

The procedure was now complete. The second spin in the centrifuge made it possible to quickly filter the rest of the supernatant and obtain the direct sample. The next step is to shake the enriched lysate until Monday. On Monday, the next step will be a spot test to determine if any arthrobacter bacteriophages have been found.